Fig. 4. Expression of Helios in RA patient groups with different disease activity and with different treatments. (a) Helios was significantly lowerd in high disease activity RA than in healthy controls (p<0.01). (b)The expression of Helios in Tregs from RA patients was compared according to whether patients were on treatments such as DMARDs (c), Steroids (d) (p>0.05) or TNFi (e) (p<0.05). The p value was measured with Mann-Whitney test. n.s., not significant, * and ** indicate p <0.05 and p <0.01, respectively.